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| Pradillon, F; Le Bris, Nadine; Shillito, B; Young, C; Gaill, F. |
| Dispersal and colonisation processes at deep-sea vents are still not fully understood, essentially because early life stages of vent species remain unknown. The polychaete worm Alvinella pompejana forms colonies on chimney walls at East Pacific Rise vent sites where the temperature can frequently exceed 20 degrees C. In vitro studies in pressure vessels showed that the early embryos tolerate temperatures in a lower range (10-14 degrees C), suggesting that they would have to escape the colony to develop. Pressure vessels offer the advantage that each parameter can be independently controlled, but they do not simulate the more complex and dynamic conditions naturally encountered at vent sites. Accordingly, in addition to incubations in pressure vessels, we... |
| Tipo: Text |
Palavras-chave: Environmental condition; H2S; Temperature tolerance; Polychaete; Pressure vessel; Embryo; Development; Hydrothermal vent. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-600.pdf |
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| Zbinden, M; Shillito, B; Le Bris, Nadine; De Montlaur, C; Roussel, Erwan; Guyot, F; Gaill, F; Cambon-bonavita, Marie-anne. |
| The Rimicaris exoculata dominates the megafauna of some of the Mid Atlantic ridge hydrothermal vent sites. This species harbors a rich community of bacterial epibionts inside its gill chamber. Literature data indicate that a single 16S rRNA phylotype dominates this epibiotic community, and is assumed to be a sulfide-oxidizing bacteria. However attempts Of Cultivation were not successful and did not allow to confirm it. The aim of our study was to test the hypothesis of sulfide oxidation in the gill chamber, by a multidisciplinary approach, using in vivo experiments at in situ pressure in the presence of sulfide, microscopic observations and a molecular survey. Morphology of microorganisms, before and after treatment, was analyzed to test the effect of... |
| Tipo: Text |
Palavras-chave: Sulfur; Methane; Iron; Intracellular granules; Hydrothermal vent shrimp; High pressure experiments. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4563.pdf |
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| Shillito, B; Le Bris, Nadine; Hourdez, S; Ravaux, J; Cottin, D; Caprais, Jean-claude; Jollivet, D; Gaill, F. |
| The shrimp Mirocaris fortunata is a hydrothermal vent species that is found at most vent-sites along the Mid-Atlantic Ridge. This endemic species is found across a hydrothermal gradient, with thermal conditions ranging from 2-9 degrees C in ambient seawater to fairly warm values of about 25 degrees C. We performed in vivo experiments on M. fortunata specimens originating from different sites and depths (850 m to 2300 m), both at atmospheric pressure and in pressurized aquaria, to characterise the upper thermal limits of this species. Atmospheric pressure results show that thermal physiology should be studied at each population's native pressure. At in situ pressure, shrimps from Menez Gwen (850 m depth) and Lucky Strike (1700 m depth) do not survive... |
| Tipo: Text |
Palavras-chave: IPOCAMP TM; Crustacea; Mirocaris fortunata; Thermal stress; Hydrothermal vent. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1240.pdf |
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| Cottin, D; Ravaux, J; Leger, N; Halary, S; Toullec, J; Sarradin, Pierre-marie; Gaill, F; Shillito, B. |
| The annelid Paralvinella grasslei is a deep-sea vent endemic species that colonizes the wall of active chimneys. We report here the first data on its thermal biology based on in vivo experiments in pressurized aquaria. Our results demonstrate that P. grasslei survives a 30 min exposure at 30 degrees C, and suggest that the upper thermal limit of this species is slightly above this temperature. The first signs of stress were noticed at 30 C, such as a significant increase in the animal's activity and the expression of HSP70 stress proteins. A preliminary investigation of the kinetics of stress protein expression surprisingly showed high levels of HSP70 proteins as late as 3.5 h after the heat shock. Finally, we provide here the first sequences for vent... |
| Tipo: Text |
Palavras-chave: IPOCAMP; Annelids; Heat stress; Stress response; Heat shock proteins; Hydrothermal vents. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4570.pdf |
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